ChAT (Choline Acetyltransferase) chicken polyclonal anti-peptide antibody-Aves Labs

Home | Register | My Account | View Cart | Logon

Catalog#	Product	Image Title	Click To View
CAT	ChAT (Choline Acetyltransferase) chicken polyclonal anti-peptide antibody	ChAT-positive neurons (red) in the neostriatum of a GFP-expressing transgenic mouse.
Order Information
Antibody Concentrations	100 ug/ml (based on Bradford assay readings using bovine serum albumin as a standard).
Volume	Regular vials contain 1000 ul of this antibody mixture; Sampler vials contain 200 ul of this mixture.
Buffer	Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline (PBS, pH 7.2) with sodium azide (0.02%, w/v) added as a preservative.
Quality Control	The antibody was analyzed by immunohistochemistry (at a concentration of 3 ug/ml) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.#F-1005) as the secondary reagent.
Recommended Dilutions	1:1000-1:2000 for immunohistochemistry and immunocytochemistry using 2% paraformaldehyde-fixed tissues or cells. Please note that these dilutions are meant to serve as starting points, and that optimal dilutions may vary.
Chickens were immunized with a synthetic peptide/keyhole limpet hemocyanin (KLH) conjugate corresponding to a region of the Choline Acetyltransferase gene product (EC 2.3.1.6) shared between the mouse (Q03059) and human (P28329) gene products. After repeated injections into the hens, immune eggs were collected, and the IgY fraction were purified from the yolks. These IgY fractions were then affinity-purified using peptide columns, the concentration of the eluate adjusted to 100 ug/ml, and the preparation was filter-sterilized. Datasheet For technical service please call 503.598.8766

Data Sheet   Featured Articles
  References
  FAQ
  Protocols

Privacy Policy Terms and Condition Return Policy Payment and Terms .

Search