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Actin Antibody (Nitrocellulose Membrane Loading Control)

Price: $115
Catalog #: ACT-1010
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Product Info

Actin Antibody (for nitrocellulose membrane loading control studies).  Chickens were immunized with purified actin protein.  After repeated injections, immune eggs were collected from the hens, and the IgY fractions were purified from the yolks.  These IgY fractions were then affinity-purified using an agarose matrix to which the whole protein was attached.  The final preparation of this product involved mixing affinity purified anti-actin antibody (final concentration of 25 ug/ml) with IgY fractions of anti-actin antibody (final concentration of 10 mg/ml) in PBS with 50% glycerol.  This preparation was then filter-sterilized.

Antibody Concentration: 10 mg/ml of purified anti-actin (purified from rabbit muscle) IgY spiked with 25 ug/ml of affinity purified anti-actin IgY (based on A280 nm assay readings)

Volume: 0.1 ml, 10.0 mg/ml

Buffer: Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline (PBS, pH 7.2) with sodium azide (0.02%, w/v) added as a preservative.

Quality Control: This antibody mixture was validated by western blotting on nitrocellulose membranes (at a concentration of 1 µg/ml) using HRP-labeled goat anti-chicken IgY (1:5000 dilution, Aves Labs Cat.# H-1004) as the secondary reagent.

Storage Temperature: -20°C

Applications: Immunocytochemistry

Antibody Type: Polyclonal

Conjugate Type: none

Special Shipping Instructions: Room Temperature

Shipping Method: Overnight Priority

Recommended Dilutions: 1:2500-1:5000 Western Blot.