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    1. Higher titres against highly conserved mammalian gene products

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Polyhistidine Anti-6XHIS (for Immunoprecipitation) Antibody

Price: $315
Catalog #: HIS-IP-1010
Data Sheet (PDF)
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Product Info

Anti-6XHIS Antibody.  Chickens were immunized with a synthetic peptide containing the 6X-HIS epitope tag – HHHHHH – conjugated to keyhole limpet hemocyanin (KLH).  After repeated injections, immune eggs were collected from the hens, and the IgY fractions were purified from the yolks.  These IgY fractions were then affinity-purified using an agarose matrix to which the cognate peptide was attached.  Eluates of this column were dialyzed against PBS, had their concentrations adjusted to 100 ug/ml, and then filter-sterilized.

Species Reactivity: n/a

Host: Chicken

Form: Affinity-purified antibodies

Storage Temperature: -20°C

Applications: Immunoprecipitation

Antibody Type: Polyclonal

Conjugate Type: none

Storage Instructions: -20°C

Special Shipping Instructions: Room Temperature

Shipping Method: Overnight Priority

Chicken anti-6XHIS Tag affinity-purified antibodies, 100 ul, 1.0 mg/ml. Recommended Dilutions: 1:5000-1:10000 for immunoprecipitation applications (IP’s and ChIP’s).