Product Catalog

  • Download our Catalog (PDF File)

  •  

    New Products

     

    Why Use Chicken IgY?

  • Here are top 5 reasons:



    1. Higher titres against highly conserved mammalian gene products

    2. Double immunostaining is easier to perform

    3. Animal-friendly -- we purify the antibodies from eggs, not serum

    4. Large quantities of antibody - faster and cheaper

    5. Nearly unlimited quantities (again, because the antibodies come from eggs)

    learn more

  •  

    Like Aves Labs on Social Media

  •  

Polyhistidine Anti-6XHIS (for westerns) Antibody

Price: $325
Catalog #: HIS-W-1040
Data Sheet (PDF)
Add to Cart
Share |

Product Info

Anti-6XHIS Antibody.  Chickens were immunized with a synthetic peptide containing the 6X-HIS epitope tag – HHHHHH – conjugated to keyhole limpet hemocyanin (KLH).  After repeated injections, immune eggs were collected from the hens, and the IgY fractions were purified from the yolks.  These IgY fractions were then affinity-purified using an agarose matrix to which the cognate peptide was attached.  Eluates of this column were dialyzed against PBS, had their concentrations adjusted to 100 ug/ml, and then filter-sterilized.

Species Reactivity: n/a

Host: Chicken

Form: Mixture of IgY fraction and affinity-purified antibodies

Storage Temperature: -20°C

Applications: Western Blot

Antibody Type: Polyclonal

Conjugate Type: none

Storage Instructions: -20°C

Special Shipping Instructions: Room Temperature

Shipping Method: Overnight Priority

Chicken anti-6XHIS Tag antibodies, 0.4 ml, 10.0 mg/ml. Recommended Dilutions: 1:5000-1:10000 Western Blot.

Reviews