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Anti-C-MYC Epitope Tag Antibody

Price: $245
Catalog #: ET-MY100
Data Sheet (PDF)
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Product Info

Anti-MYC Epitope Tag.  Chickens were immunized with a synthetic peptide containing the human c-myc epitope tag – EQKLISEEDL – conjugated to keyhole limpet hemocyanin (KLH).  The sequence of this peptide corresponds to residues #425-434 of the human c-myc proto-oncogene protein (NCBI NP_002458.2).  After repeated injections, immune eggs were collected from the hens, and the IgY fractions were purified from the yolks.  These IgY fractions were then affinity-purified using an agarose matrix to which the cognate peptide was attached.  Eluates of this column were dialyzed against PBS, had their concentrations adjusted to 100 ug/ml, and then filter-sterilized.

Species Reactivity: n/a

Host: Chicken

Form: Affinity Purified

Storage Temperature: 4°C

Applications: ELISA, Western Blot

Antibody Type: Polyclonal

Conjugate Type: none

Storage Instructions: 4°C

Special Shipping Instructions: Room Temperature

Shipping Method: Overnight Priority

Affinity purified chicken anti-c-myc epitope tag antibodies, 1.0 ml, 0.1 mg/ml. Recommended Dilutions: 1:500 Western Blot, 1:250 Immunocytochemistry, 1:250 Immunoprecipitation.