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Anti-DYKDDDDK Epitope Tag/FLAG Antibody

Price: $245
Catalog #: ET-DY100
Data Sheet (PDF)
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Product Info

Anti-DYKDDDDK Epitope Tag.  Chickens were immunized with a synthetic peptide containing the DYKDDDDK sequence conjugated to keyhole limpet hemocyanin (KLH).  The sequence of this peptide corresponds to the FLAG® epitope tag.  After repeated injections, immune eggs were collected from the hens, and the IgY fractions were purified from the yolks.  These IgY fractions were then affinity purified using an agarose matrix to which the cognate peptide was attached.  Eluates of this column were dialyzed against PBS, had their concentrations adjusted to 100 ug/ml, and then filter-sterilized.

Species Reactivity: n/a

Host: Chicken

Form: Affinity Purified

Storage Temperature: 4°C

Applications: ELISA, Western Blot, IHC, ICC

Antibody Type: Polyclonal

Conjugate Type: none

Storage Instructions: 4°C

Special Shipping Instructions: Room Temperature

Shipping Method: Overnight Priority

Affinity purified chicken anti-DYKDDDDK or FLAG epitope tag antibodies, 1.0 ml, 0.1 mg/ml. Recommended Dilutions: 1:1000 Western Blot, 1:500 Immunocytochemistry, 1:500 Immunoprecipitation.