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    1. Higher titres against highly conserved mammalian gene products

    2. Double immunostaining is easier to perform

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Coronin 1a

Price: $399
Catalog #: COR
Data Sheet (PDF)
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Product Info

Antibody Concentrations: 100 µg/ml (based on Bradford assay readings using bovine serum albumin as a standard).

Volume: Regular vials contain 1000 µl of this antibody mixture; Sampler vials contain 200 µl of this mixture.

Buffer: Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline (PBS, pH 7.2) with sodium azide (0.02%, w/v) added as a preservative.

Quality Control: The antibody was analyzed by immunohistochemistry (at a concentration of 3 µg/ml) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.#F-1005) as the secondary reagent.

Recommended Dilutions: 1:1000-1:2000 for immunohistochemistry and immunocytochemistry using 2% paraformaldehyde-fixed tissues or cells. Please note that these dilutions are meant to serve as starting points, and that optimal dilutions may vary.

Description: Chickens were immunized with a synthetic peptide/keyhole limpet hemocyanin (KLH) conjugate corresponding to a region of the Coronin 1a gene product shared between the mouse (NM_009898) and human (NM_007074) gene products. After repeated injections into the hens, immune eggs were collected, and the IgY fraction were purified from the yolks. These IgY fractions were then affinity-purified using peptide columns, the concentration of the eluate adjusted to 100 µg/ml, and the preparation was filter-sterilized.