Product Catalog

  • Download our Catalog (PDF File)

  •  

    Try a Sampler Kit

     

    Why Use Chicken IgY?

  • Here are top 5 reasons:



    1. Higher titres against highly conserved mammalian gene products

    2. Double immunostaining is easier to perform

    3. Animal-friendly -- we purify the antibodies from eggs, not serum

    4. Large quantities of antibody - faster and cheaper

    5. Nearly unlimited quantities (again, because the antibodies come from eggs)

    learn more

  •  

    Like Aves Labs on Social Media

  •  

Proteolipid Protein (PLP)

Price: $385
Catalog #: PLP
Data Sheet (PDF)
In this tissue section through an e13 mouse brain, PLP (green staining) can be seen in immature oligodendrocytes of white matter tracts. DAPI (blue staining) allows visualization of nuclei.
Add to Cart
Share |

Product Info

Antibody Concentrations: 100 µg/ml (based on Bradford assay readings using bovine serum albumin as a standard).

Volume: Regular vials contain 1000 µl of this antibody; Sampler vials contain 100 µl of this antibody.

Buffer: Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline (PBS, pH 7.2) with sodium azide (0.02%, w/v) added as a preservative.

Quality Control: Antibodies were analyzed by immunohistochemistry (at a dilution of 1:2000) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.# F-1005) as the secondary reagent.

Recommended Dilutions: 1:1000-1:2000 for immunohistochemistry and immunocytochemistry using 2% paraformaldehyde-fixed tissues or cells. Please note that these dilutions are meant to serve as starting points, and that optimal dilutions may vary.

Description: Chickens were immunized with a synthetic peptide / keyhole limpet hemocyanin (KLH) conjugate. The sequence of the synthetic peptide was shared between the human and mouse gene products. After repeated injections into the hens, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, the concentration of the eluate adjusted to 100 µg/ml, and the preparation was filter sterilized.

Reviews