Glial Fibrillary Acidic Protein (GFAP)
µµAntibody Concentrations: 2.0 mg/ml
Volume: Regular vials contain 200 µl of this antibody mixture.
Buffer: Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline (PBS, pH 7.2) with sodium azide (0.02%, w/v) added as a preservative.
Quality Control: Immunohistochemistry (at a dilution of 1:5000) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.# F-1005) as the secondary reagent.
Recommended Dilutions: 1:2000-1:5000 for immunohistochemistry and immunocytochemistry using 2% paraformaldehyde-fixed cells. 1:5000-1:10000 for western blotting. Please note that these dilutions are meant to serve as starting points, and that optimal dilutions may vary.
Chickens were immunized with recombinant GFAP. After repeated injections into the hens, immune eggs were collected, and the IgY fractions were purified from the yolks. Finally, sodium azide was added to a concentration of 0.02% (w/v), the IgY concentrations were adjusted to 2.0 mg/ml, the preparation was filter-sterilized, and then aliquots prepared.