Peripherin
Antibody Concentrations: 2.0 mg/ml.
Volume: Regular vials contain 200 µl of this antibody preparation.
Buffer: Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline (PBS, pH 7.2) with sodium azide (0.02%, w/v) added as a preservative.
Quality: Control Antibodies were analyzed by immunohistochemistry (at a dilution of 1:5000) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.# F-1005) as the secondary reagent.
Recommended Dilutions: 1:2000-1:5000 for immunohistochemistry and immunocytochemistry using 2% paraformaldehyde-fixed tissues or cells. 1:5000-1:10000 for western blotting. Note that these dilutions are meant to serve as starting points, and that optimal dilutions may vary.
Chickens were immunized with purified recombinant full-length rat peripherin protein (57 kDa). After repeated injections into the hens, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions are diluted 1:10 with 1% bovine serum albumin (to reduce absorption) in phosphate-buffered isotonic saline solution (PBS) with 0.02% sodium azide (as a preservative).