PAP (Prostatic Acid Phosphatase) – A Pain System Marker 200µl
About PAP: Mouse PAP is a 43,698 dalton protein (381 amino acids; NCBI accession numberAAF23171) associated with prostatic cancer cells, as well as primary afferent sensory neurons involved in the pain pathway. This protein is an enzyme that dephosphorylates adenosine monophosphate (AMP) in the dorsal horn gray matter of the spinal cord, generating free adenosine. Injections of PAP into the dorsal horn of experimental mice has been shown to decrease pain perception by acting in an antinociceptive, antihyperalgesic, and antiallodynic fashion.
About PAP Antibodies: Recombinant mouse PAP protein was expressed using a baculoviral-delivery system. Antibodies against this protein were prepared by injecting purified recombinant PAP into laying hens, and purifying the IgY fraction from eggs collected from hyper-immunized hens.
IgY Concentrations: 10 mg/ml of chicken total IgY antibody supplemented with 20 μg/ml of affinity purified antibody. The vehicle is phosphate-buffered isotonic saline (PBS) (50% by volume) and glycerol (50% by volume) supplemented with 0.02% sodium azide as a preservative.
Applications: Immunohistochemistry (1:500-1:1000 recommended dilution)