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Anti-Arl13b (N295B/66) – Purified

Price: $319
Catalog #: 75-287
Data Sheet (PDF)
Immunofluorescence staining of adult rat brain sections with N295B/66 (red, cilia) and N147/6 (green) TC supe.
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Product Info

OVERVIEW

Form: Purified
Produced by in vitro bioreactor culture of hybridoma lines followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody at 1mg/mL.

Volume:
100 ┬ÁL

Concentration:
1 mg/mL

Brand:
NeuroMab

Antibody Type:
Monoclonal

Host Species:
Mouse
Antibody Isotype:
IgG2a

Species Reactivity:
Mouse, Rat

Applications:
ICC, IHC, WB

Immunogen:
Fusion protein amino acids 208-427 (C-terminus) of mouse Arl13b (also known as ADP-ribosylation factor-like protein 13B, ADP-ribosylation factor-like protein 2-like 1, ARL2-like protein 1 and Arl2l1, accession number Q640N2)
Rat: 85% identity (189/220 amino acids identical)
Human: 68% identity (154/224 amino acids identical)
<40% identity with Arl13a

Cross Reactivity:
Does not cross-react with Arl13a (based on KO validation results)

Protein Name(s):
ADP-ribosylation factor-like protein 13B (ADP-ribosylation factor-like protein 2-like 1) (ARL2-like protein 1) (Protein hennin)

Gene Name(s):
Arl13b Arl2l1

Antibody Registry ID:
Purified: AB_11001586

ANTIBODY VALIDATION AND APPLICATION NOTES
This antibody has been validated using the following assays:
Knockout Validation:
This antibody has been knockout-validated in mouse brain (by Western blot and/or immunohistochemistry).

Western Blot:
This antibody recognizes a single immunoreactive band of expected molecularweight when used to probe brain lysate.

Immunocytochemistry:
This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Immunohistochemistry:
This antibody shows the expected immunoperoxidase-diaminobenzidine/immunofluorescence staining pattern when used to stain brain sections.
Expected Banding Pattern:
50 kDa

QUALITY CONTROL
The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.
Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.

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