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OVERVIEW
Form: Purified
Produced by in vitro bioreactor culture of hybridoma lines followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody at 1mg/mL.
Volume:
100 µL
Concentration:
1 mg/mL
Brand:
NeuroMab
Antibody Type:
Monoclonal
Host Species:
Mouse
Antibody Isotype:
IgG1
Species Reactivity:
Human, Mouse, Rat
Applications:
ICC, IHC, WB
Immunogen:
Synthetic peptide amino acids 411-422 (KTVEMRDGEVIK) of human GFAP (also known as Glial fibrillary acidic protein, accession number P14136)
Rat: 100% identity (12/12 amino acids identical)
Mouse: 100% identity (12/12 amino acids identical) >50% identity with other proteins (Vimentin, Desmin, Tumor protein p73 and Peripherin)
Cross Reactivity:
Cross-reacts with GFAP-R416W and other GFAP mutant proteins Does not cross-react with other proteins (based on KO validation results)
Protein Name(s):
Glial fibrillary acidic protein (GFAP)
Gene Name(s):
GFAP
Antibody Registry ID:
Purified: AB_10672299
ANTIBODY VALIDATION AND APPLICATION NOTES
This antibody has been validated using the following assays:
Knockout Validation:
This antibody has been knockout-validated in mouse brain (by Western blot and/or immunohistochemistry).
Western Blot:
This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.
Immunocytochemistry:
This antibody shows the expected staining pattern when used to stain COS cells over-expressing target.
Immunohistochemistry:
This antibody shows the expected immunoperoxidase-diaminobenzidine/immunofluorescence staining pattern when used to stain brain sections.
Expected Banding Pattern:
50 kDa
QUALITY CONTROL
The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.
Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.