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Anti-GFAP (N206A/8) – TC Supernatant

Price: $319
Catalog #: 73-240
Data Sheet (PDF)
Adult WT and KO mouse hippocampus immunofluorescence with N206A/8 (green) and KC rabbit antiKv2.1 (red). Brain samples courtesy of Albee Messing, University of Wisconsin at Madison.
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Product Info

OVERVIEW

Form: TC Supernatant
Hybridoma tissue-culture supernatant (5mL) generally containing 20-60 ug/mL monoclonal antibody (mAb). Supernatant is suitable for most applications, including Western Blot, immunocytochemistry, immunohistochemistry, etc., and is a very economical choice compared to competitor offerings. For applications requiring higher antibody concentration or purity (such as immunoprecipitation, microinjection, or conjugation) it is recommended that you purchase the purified form of the antibody (supplied at 1mg/mL).

Volume:
5 mL

Concentration:
Lot dependent

Brand:
NeuroMab

Antibody Type:
Monoclonal

Host Species:
Mouse

Antibody Isotype:
IgG1

Species Reactivity:
Human, Mouse, Rat

Applications:
ICC, IHC, WB

Immunogen:
Synthetic peptide amino acids 411-422 (KTVEMRDGEVIK) of human GFAP (also known as Glial fibrillary acidic protein, accession number P14136)
Rat: 100% identity (12/12 amino acids identical)
Mouse: 100% identity (12/12 amino acids identical) >50% identity with other proteins (Vimentin, Desmin, Tumor protein p73 and Peripherin)

Cross Reactivity:
Cross-reacts with GFAP-R416W and other GFAP mutant proteins Does not cross-react with other proteins (based on KO validation results)

Protein Name(s):
Glial fibrillary acidic protein (GFAP)

Gene Name(s):
GFAP

Antibody Registry ID:
AB_10672298

ANTIBODY VALIDATION AND APPLICATION NOTES
This antibody has been validated using the following assays:
Knockout Validation:
This antibody has been knockout-validated in mouse brain (by Western blot and/or immunohistochemistry).

Western Blot:
This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.

Immunocytochemistry:
This antibody shows the expected staining pattern when used to stain COS cells over-expressing target.

Immunohistochemistry:
This antibody shows the expected immunoperoxidase-diaminobenzidine/immunofluorescence staining pattern when used to stain brain sections.
Expected Banding Pattern:
50 kDa

QUALITY CONTROL
The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.
Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.

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