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Anti-GluN2B/NR2B glutamate receptor (N59/36) – TC Supernatant

Price: $319
Catalog #: 73-101
Data Sheet (PDF)
Adult rat hippocampus immunohistochemistry
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Product Info

OVERVIEW

Form: TC Supernatant
Hybridoma tissue-culture supernatant (5mL) generally containing 20-60 ug/mL monoclonal antibody (mAb). Supernatant is suitable for most applications, including Western Blot, immunocytochemistry, immunohistochemistry, etc., and is a very economical choice compared to competitor offerings. For applications requiring higher antibody concentration or purity (such as immunoprecipitation, microinjection, or conjugation) it is recommended that you purchase the purified form of the antibody (supplied at 1mg/mL).

Volume:
5 mL

Concentration:
Lot dependent

Brand:
NeuroMab

Antibody Type:
Monoclonal

Host Species:
Mouse

Antibody Isotype:
IgG2b

Species Reactivity:
Human, Mouse, Rat

Applications:
IHC, IP, WB

Immunogen:
Fusion protein amino acids 20-271 (extracellular N-terminus) of rat NR2B (also known as N-methyl D-aspartate/NMDA receptor subtype 2B or accession number Q00960)
Human: 99% identity (250/252 amino acids identical)
Mouse: 99% identity (250/252 amino acids identical)

Cross Reactivity:
Does not cross-react with GluN2A/NR2A

Protein Name(s):
Glutamate receptor ionotropic, NMDA 2B (GluN2B) (Glutamate [NMDA] receptor subunit epsilon-2) (N-methyl D-aspartate receptor subtype 2B) (NMDAR2B) (NR2B)

Gene Name(s):
Grin2b

Antibody Registry ID:
AB_10672980

ANTIBODY VALIDATION AND APPLICATION NOTES
This antibody has been validated using the following assays:
Knockout Validation:
This antibody has been knockout-validated in mouse brain (by Western blot and/or immunohistochemistry).

Western Blot:
This antibody recognizes a single immunoreactive band of expected molecularweight when used to probe brain lysate.

Immunocytochemistry:
This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Immunohistochemistry:
This antibody shows the expected immunoperoxidase-diaminobenzidine/immunofluorescence staining pattern when used to stain brainsections.

Expected Banding Pattern:
166 kDa

QUALITY CONTROL
The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.
Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.

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