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Anti-Kv2.1 K+ channel (K89/34) – TC Supernatant

Price: $319
Catalog #: 73-014
Data Sheet (PDF)
Adult rat hippocampus immunohistochemistry
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Product Info

OVERVIEW

Form: TC Supernatant
Hybridoma tissue-culture supernatant (5mL) generally containing 20-60 ug/mL monoclonal antibody (mAb). Supernatant is suitable for most applications, including Western Blot, immunocytochemistry, immunohistochemistry, etc., and is a very economical choice compared to competitor offerings. For applications requiring higher antibody concentration or purity (such as immunoprecipitation, microinjection, or conjugation) it is recommended that you purchase the purified form of the antibody (supplied at 1mg/mL).

Volume:
5 mL

Concentration:
Lot dependent

Brand:
NeuroMab

Antibody Type:
Monoclonal

Host Species:
Mouse

Antibody Isotype:
IgG1

Species Reactivity:
Human Mouse Rat

Applications:
IHC IP WB

Immunogen:
Synthetic peptide amino acids 837-853 (HMLPGGGAHGSTRDQSI, cytoplasmic Cterminus) of rat Kv2.1 (also known as Potassium voltage-gated channel subfamily B member 1, Delayed rectifier potassium channel 1, DRK1, Kcnb1 and Shab, accession number P15387)
Mouse: 100% identity (17/17 amino acids identical)
Human: 88% identity (15/17 amino acids identical, RVLPGGGAHGSTRDQSI)

Cross Reactivity:
No cross-reactivity against rat Kv2.2

Protein Name(s):
Potassium voltage-gated channel subfamily B member 1 (Delayed rectifier potassium channel 1) (DRK1) (Voltage-gated potassium channel subunit Kv2.1)

Gene Name(s):
Kcnb1

Antibody Registry ID:
AB_10672253

ANTIBODY VALIDATION AND APPLICATION NOTES
This antibody has been validated using the following assays:
Knockout Validation:
This antibody has been knockout-validated in mouse brain (by Western blot and/or immunohistochemistry).

Western Blot:
This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe lysate from COS cells overexpressing target.

This antibody recognizes a single immunoreactive band of expected molecularweight when used to probe brain lysate.

Immunocytochemistry:
This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Immunohistochemistry:
This antibody shows the expected immunoperoxidase-diaminobenzidine/immunofluorescence staining pattern when used to stain brain sections.
Expected Banding Pattern:
105-125 kDa(varies with cell background due to phosphorylation)

QUALITY CONTROL
The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.
Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.

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