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Anti-Pan-Neurofascin (extracellular) (A12/18) – TC Supernatant

Price: $319
Catalog #: 73-172
Data Sheet (PDF)
Adult rat brain immunohistochemistry
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Product Info

OVERVIEW

Form: TC Supernatant
Hybridoma tissue-culture supernatant (5mL) generally containing 20-60 ug/mL monoclonal antibody (mAb). Supernatant is suitable for most applications, including Western Blot, immunocytochemistry, immunohistochemistry, etc., and is a very economical choice compared to competitor offerings. For applications requiring higher antibody concentration or purity (such as immunoprecipitation, microinjection, or conjugation) it is recommended that you purchase the purified form of the antibody (supplied at 1mg/mL).

Volume:
5 mL

Concentration:
Lot dependent

Brand:
NeuroMab

Antibody Type:
Monoclonal

Host Species:
Mouse

Antibody Isotype:
IgG2a

Species Reactivity:
Human, Rat

Applications:
IHC, IP, WB

Immunogen:
Lipid raft fractions isolated from adult rat brain. Target identified as Neurofascin by: – binding to NF-186-tranfected COS cells- recognition of NF155 and NF186 but not other highly related L1 CAMs like NrCAM- immunoprecipitation of NF155 and NF186 and confirmation by mass spectrometry – loss of immunostaining against NF186-shRNA knockdown samples
Rat: amino acids 25-1110 (extracellular domain common to NF155 and NF186)
Mouse: 98% identity (1074/1086 amino acids identical)
Human: 79% identity (955/1195 amino acids identical)
This NeuroMab antibody is considered “Restricted” and is therefore not available for commercial re-distribution on a for-profit basis.

Cross Reactivity:
None Reported

Protein Name(s):
Neurofascin NF-155 NF-186

Gene Name(s):
Nfasc

Antibody Registry ID:
AB_10671311

ANTIBODY VALIDATION AND APPLICATION NOTES
This antibody has been validated using the following assays:
Western Blot:
This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.

Immunocytochemistry:
This antibody shows the expected staining pattern when used to stain COS cells over-expressing target.

Immunohistochemistry:
This antibody shows the expected immunoperoxidase-diaminobenzidine/immunofluorescence staining pattern when used to stain brain sections.
Expected Banding Pattern:
155, 186 kDa

QUALITY CONTROL
The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.
Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.

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