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Anti-RGS14 (N133/21) – Purified

Price: $319
Catalog #: 75-170
Data Sheet (PDF)
Adult rat hippocampus immunohistochemistry
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Product Info

OVERVIEW

Form: Purified
Produced by in vitro bioreactor culture of hybridoma lines followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody at 1mg/mL.

Volume:
100 ┬ÁL

Concentration:
1 mg/mL

Brand:
NeuroMab

Antibody Type:
Monoclonal

Host Species:
Mouse

Antibody Isotype:
IgG2a

Species Reactivity:
Mouse, Rat

Applications:
ICC, IHC, WB

Immunogen:
Fusion protein amino acids 1-544 (full-length) of rat RGS14 (also known as Regulator of G-protein signaling 14, RAP1/RAP2-interacting protein and RPIP1, accession number O08773)
Mouse: 95% identity (524/547 amino acids identical)
Human: 86% identity (475/547 amino acids identical)
45% identity with RGS12 and other RGS proteins
Epitope mapped to within amino acids 444-490 (between 2nd Ras-binding
GoLoco domains, PDAKTREASSIPPCRSQGCLPRTQTKDSHLPPLSSSLSVEDASGSTG, 2014 Evans et al J Comp Neurol)
Mouse: 85% identity (40/47 amino acids identical)
Human: 51% identity (24/47 amino acids identical)

Cross Reactivity:
Does not react with RGS12

Protein Name(s):
Regulator of G-protein signaling 14 (RGS14)

Gene Name(s):
Rgs14

Antibody Registry ID:
AB_10698026

ANTIBODY VALIDATION AND APPLICATION NOTES
This antibody has been validated using the following assays:
Knockout Validation:
This antibody has been knockout-validated in mouse brain (by Western blot and/or immunohistochemistry).

Western Blot:
This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.

Immunocytochemistry:
This antibody shows the expected staining pattern when used to stain COS cells over-expressing target.

Immunohistochemistry:
This antibody shows the expected immunoperoxidase-diaminobenzidine/immunofluorescence staining pattern when used to stain brain sections.

Expected Banding Pattern:
60 kDa

QUALITY CONTROL
The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.
Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.

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