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Anti-Slo1 maxi-K+ channel (L6/60) – Purified

Price: $319
Catalog #: 75-022
Data Sheet (PDF)
Top: adult rat hippocampus immunohistochemistry; Bottom: immunofluorescence staining of hippocampal CA3 of adult WT and Slo1 KO mice with L6/60 (red) and Kv2.1 rabbit polyclonal (green).
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Product Info

OVERVIEW

Form: Purified
Produced by in vitro bioreactor culture of hybridoma lines followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody at 1mg/mL.

Volume:
100 ┬ÁL

Concentration:
1 mg/mL

Brand:
NeuroMab

Antibody Type:
Monoclonal

Host Species:
Mouse

Antibody Isotype:
IgG2a

Species Reactivity:
Human, Mouse, Rat, Zebrafish

Applications:
IHC, IP, WB

Immunogen:
Fusion protein amino acids 690-1196 (cytoplasmic C-terminus) of mouse Slo1 (accession number AAA39746)
Human: 99% identity
Rat: 100% identity
Epitope mapped within amino acids 690-715 by Dr. D. Levy (Univ of Chicago); segment is 100% conserved in mouse, rat, human, horse, dog, pig, cow, Rhesus monkey, rabbit.

Cross Reactivity:
None Reported

Protein Name(s):
Calcium-activated potassium channel subunit alpha-1 (BK channel) (BKCA alpha) (Calcium-activated potassium channel, subfamily M subunit alpha-1) (K(VCA)alpha) (KCa1.1) (Maxi K channel) (MaxiK) (Slo-alpha) (Slo1) (mSlo1) (Slowpoke homolog) (Slo homolog) (mSlo)

Gene Name(s):
Kcnma1 Kcnma

Antibody Registry ID:
Purified: AB_2249538

ANTIBODY VALIDATION AND APPLICATION NOTES
This antibody has been validated using the following assays:
Knockout Validation:
This antibody has been knockout-validated in mouse brain (by Western blot and/or immunohistochemistry).

Western Blot:
This antibody recognizes a single immunoreactive band of expected molecularweight when used to probe brain lysate.

Immunocytochemistry:
This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Immunohistochemistry:
This antibody shows the expected immunoperoxidase-diaminobenzidine/immunofluorescence staining pattern when used to stain brain sections.
Expected Banding Pattern:
110-130 kDa

QUALITY CONTROL
The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.
Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.

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