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    Why Use Chicken IgY?

  • Here are top 5 reasons:

    1. Higher titres against highly conserved mammalian gene products

    2. Double immunostaining is easier to perform

    3. Animal-friendly -- we purify the antibodies from eggs, not serum

    4. Large quantities of antibody - faster and cheaper

    5. Nearly unlimited quantities (again, because the antibodies come from eggs)

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Beta-Amyloid Peptide (N-terminus)

Price: $399
Catalog #: ABN
Data Sheet (PDF)
Beta-Amyloid-positive “neuritic plaque” in cerebral cortex as seen in a post-mortum specimen taken from an
Alzheimer’s disease patient.  Picture courtesy of Dr. Randy Woltjer, Oregon Health & Sciences University.
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Product Info

Antibody Concentration:  100 ug/ml (based on A280)

Volume: Regular vials contain 1000 ul; Sampler vials contain 200 ul.

Buffer: Phosphate-buffered (10 mM) isotonic saline (0.9%, w.v) with sodium azide (0.02%, w/v) added as a preservative.

Quality Control: Immunohistochemistry (at  a concentration of 100 ug/ml) using HRP-labeled goat anti-chicken IgY (1:1000 dilution, Aves Labs Cat# F-1005) as the secondary reagent.  Please note that these dilutions are meant to serve as starting points, and that optimal dilutions may vary.

Description: Chickens were immunized with a synthetic peptide (corresponding to DAE FRH DSG YEV HHQ KL, residues #1-17 of the amyloid beta-peptide) coupled to keyhole limpet hemocyanin (KLH).  After repeated injections, immune eggs were collected, and the IgY fractions were purified from the yolks.  These IgY fractions were then affinity-purified using a peptide column to which the same peptide was attached.  Finally, the antibody concentration was adjusted to 100 ug/ml and filter-sterilized.