Product Catalog

  • Download our Catalog (PDF File)

  •  

    Try a Sampler Kit

     

    Why Use Chicken IgY?

  • Here are top 5 reasons:



    1. Higher titres against highly conserved mammalian gene products

    2. Double immunostaining is easier to perform

    3. Animal-friendly -- we purify the antibodies from eggs, not serum

    4. Large quantities of antibody - faster and cheaper

    5. Nearly unlimited quantities (again, because the antibodies come from eggs)

    learn more

  •  

    Like Aves Labs on Social Media

  •  

Choline Acetyltransferase (ChAT)

Price: $399
Catalog #: CAT
Data Sheet (PDF)
ChAT-positive neurons (red) in the neostriatum of a GFP-expressing transgenic mouse.
Add to Cart
Share |

Product Info

Antibody Concentrations: 100 µg/ml (based on Bradford assay readings using bovine serum albumin as a standard).

Volume: Regular vials contain 1000 µl of this antibody mixture; Sampler vials contain 200 µl of this mixture.

Buffer: Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline (PBS, pH 7.2) with sodium azide (0.02%, w/v) added as a preservative.

Quality Control: The antibody was analyzed by immunohistochemistry (at a concentration of 3 µg/ml) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.#F-1005) as the secondary reagent.

Recommended Dilutions: 1:1000-1:2000 for immunohistochemistry and immunocytochemistry using 2% paraformaldehyde-fixed tissues or cells. Please note that these dilutions are meant to serve as starting points, and that optimal dilutions may vary.

Description: Chickens were immunized with a synthetic peptide / keyhole limpet hemocyanin (KLH) conjugate.  The peptide corresponded to a region of the Choline Acetyltransferase gene product shared between the human (P_066264, NCBI) and mouse (Q03059, NCBI) sequences.  After repeated injections, immune eggs were collected, the IgY fractions were purified from the yolks, and then affinity-purified using a peptide column.  The concentrations of the eluates were then adjusted to 100 ug/ml, and the preparation was filter-sterilized.

Reviews