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    1. Higher titres against highly conserved mammalian gene products

    2. Double immunostaining is easier to perform

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    4. Large quantities of antibody - faster and cheaper

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Doublecortin

Price: $399
Catalog #: DCX
Data Sheet (PDF)
Doublecortin-staining of cochlear neurons in the cochlear ganglion  of an adult mouse brain (green staining); Red staining is neurofilament immunoreactivity, as seen using a rabbit antibody.
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Product Info

Antibody Concentrations: 200 µg/ml anti-peptide antibody mixture (based on A280 assay readings)

Volume: Regular vials contain 1000 µl of this antibody mixture; Sampler vials contain 200 µl of this mixture.

Buffer: Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline (PBS, pH 7.2) with sodium azide (0.02%, w/v) added as a preservative.

Quality Control: This anti-peptide antibody mixture was validated by immunohistochemistry (at a concentration of 2 µg/ml) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.# F-1005) as the secondary reagent.

Recommended Dilutions: 1:1000-1:2000 for immunohistochemistry and immunocytochemistry using 2% paraformaldehyde-fixed tissues or cells. Note that these dilutions are meant to serve as starting points, and that optimal dilutions may vary.

Description: Chickens were immunized with two synthetic peptide / keyhole limpet hemocyanin (KLH) conjugates.  These synthetic peptides corresponded to different regions of the doublecortin gene product, but are shared between the human (CAA06617.1, NCBI) and mouse (AAT58219.1, NCBI) sequences. After repeated injections, immune eggs were collected, and the IgY fractions were purified from the yolks.  These IgY fractions were then affinity-purified using a peptide column, and the concentrations of the eluates adjusted to 200 ug/ml.  Finally, equal volumes of these affinity-purified anti-peptide antibodies were mixed, and the preparation was filter-sterilized.

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