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    Why Use Chicken IgY?

  • Here are top 5 reasons:



    1. Higher titres against highly conserved mammalian gene products

    2. Double immunostaining is easier to perform

    3. Animal-friendly -- we purify the antibodies from eggs, not serum

    4. Large quantities of antibody - faster and cheaper

    5. Nearly unlimited quantities (again, because the antibodies come from eggs)

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Glutamic Acid Decarboxylase-67 (GAD 67)

Price: $399
Catalog #: GAD
Data Sheet (PDF)
A tissue section through an adult mouse brain showing GAD-67 (red staining) in basket cells of the hippocampal formation. Green staining is autofluorescence from green fluorescent protein (GFP) expressed in this transgenic mouse. Picture courtesy of Dr. Felix Eckenstein, University of Vermont.
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Product Info

Antibody Concentrations: 100 µg/ml (based on Bradford assay readings using bovine serum albumin as a standard).

Volume: Regular vials contain 1000 µl of this antibody mixture; Sampler vials contain 200 µl of this mixture.

Buffer: Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline (PBS, pH 7.2) with sodium azide (0.02%, w/v) added as a preservative.

Quality Control: The antibody was analyzed by immunohistochemistry (at a dilution of 1:1000) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.#F-1005) as the secondary reagent.

Recommended Dilutions: 1:1000-1:2000 for immunohistochemistry and immunocytochemistry using 2% paraformaldehyde-fixed tissues or cells. Please note that these dilutions are meant to serve as starting points, and that optimal dilutions may vary.

Description: Chickens were immunized with a synthetic peptide / keyhole limpet hemocyanin (KLH) conjugate.  This synthetic peptide corresponded to a region near the C-terminus of this gene product, and was 100% conserved between the human (Q99259, NCBI), mouse (P48318, NCBI) and rat (NP_058703, NCBI) gene products. After repeated injections into the hens, immune eggs were collected, and the IgY fractions were purified from the yolks.  These IgY fractions were then affinity-purified using a peptide column, the concentrations of the eluate adjusted to 100 ug/ml, and the preparation was filter-sterilized.

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