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    Why Use Chicken IgY?

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    1. Higher titres against highly conserved mammalian gene products

    2. Double immunostaining is easier to perform

    3. Animal-friendly -- we purify the antibodies from eggs, not serum

    4. Large quantities of antibody - faster and cheaper

    5. Nearly unlimited quantities (again, because the antibodies come from eggs)

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Growth-Associated Protein, 43 kDa (GAP-43)

Price: $245
Catalog #: GAP43
Data Sheet (PDF)
GAP-43-staining of cultured cortical neurons purified from a neonatal mouse brain (green staining):  Red staining corresponds to a rabbit anti-alpha II spectrin; Blue staining corresponds to the Hoechst nuclear stain.
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Product Info

Antibody Concentrations: 2.0 mg/ml

Volume: Regular vials contain 200 µl of this antibody mixture.

Buffer: Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline (PBS, pH 7.2) with sodium azide (0.02%, w/v) added as a preservative.

Quality Control: Immunohistochemistry (at a dilution of 1:5000) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.# F-1005) as the secondary reagent.

Recommended Dilutions: 1:500-1:1000 for immunohistochemistry and immunocytochemistry using 2% paraformaldehyde-fixed cells. 1:1000-1:5000 for western blotting. Please note that these dilutions are meant to serve as starting points, and that optimal dilutions may vary.

Description: Chickens were immunized with a synthetic peptide corresponding to the C-terminal region of mouse GAP-43 coupled to keyhole limpet hemocyanin.  After repeated injections, immune eggs were collected, the IgY fractions were purified from the yolks, and the IgY concentration adjusted to 20 mg/ml.  This preparation was then diluted 1:10 with PBS containing bovine serum albumin as a carrier.  Finally, the antibody preparation was filter-sterilized.

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