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    1. Higher titres against highly conserved mammalian gene products

    2. Double immunostaining is easier to perform

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Glutamate Receptor (Metabotropic) Type 1 (mGluR1)

Price: $399
Catalog #: ER1
Data Sheet (PDF)
mGluR1-staining of cortical neurons in an adult transgenic mouse brain (red staining); Green staining is GFP-immunoreactivity for a subpopulation of cortical neurons.
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Product Info

Antibody Concentrations: 300 µg/ml anti-peptide antibody mixture (based on Bradford assay readings using bovine serum albumin as a standard)

Volume: Regular vials contain 1000 µl of this antibody mixture; Sampler vials contain 200 µl of this mixture.

Buffer: Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline (PBS, pH 7.2) with sodium azide (0.02%, w/v) added as a preservative.

Quality Control: This anti-peptide antibody mixture was validated by immunohistochemistry (at a concentration of 3 µg/ml) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.# F-1005) as the secondary reagent.

Recommended Dilutions: 1:1000-1:2000 for immunohistochemistry and immunocytochemistry using 2% paraformaldehyde-fixed tissues or cells. Note that these dilutions are meant to serve as starting points, and that optimal dilutions may vary.

Description: Chickens were immunized with three synthetic peptide / keyhole limpet hemocyanin (KLH) conjugates.  These synthetic peptides corresponded to different regions of the metabotropic glutamate receptor type 1 gene product, but are shared between the human (NP_000829.2, NCBI) and mouse (NP_058672.1, NCBI) sequences. After repeated injections, immune eggs were collected, and the IgY fractions were purified from the yolks.  These IgY fractions were then affinity-purified using a peptide column, and the concentrations of the eluates adjusted to 300 ug/ml.  Finally, equal volumes of these affinity-purified anti-peptide antibodies were mixed, and the preparation was filter-sterilized.