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    1. Higher titres against highly conserved mammalian gene products

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Glutamate Receptor (Metabotropic) Type 2 and Type 3 (mGluR2/mGluR3)

Price: $399
Catalog #: ER2/3
Data Sheet (PDF)
mGluR2-staining of pyramidal neurons in the hippocampal formation of an adult transgenic mouse brain (red staining); Green staining is GFP-immunoreactivity for a subpopulation of cortical neurons.
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Product Info

Antibody Concentrations: 200 µg/ml anti-peptide antibodies (based on Bradford assay readings using bovine serum albumin as a standard)

Volume: Regular vials contain 1000 µl of this antibody preparation; Sampler vials contain 200 µl of this preparation.

Buffer: Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline (PBS, pH 7.2) with sodium azide (0.02%, w/v) added as a preservative.

Quality Control: This anti-peptide antibody preparation was validated by immunohistochemistry (at a concentration of 1 µg/ml) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.# F-1005) as the secondary reagent.

Recommended Dilutions: 1:1000-1:2000 for immunohistochemistry and immunocytochemistry using 2% paraformaldehyde-fixed tissues or cells. Note that these dilutions are meant to serve as starting points, and that optimal dilutions may vary.

Description: Chickens were immunized with two synthetic peptide / keyhole limpet hemocyanin (KLH) conjugates. These synthetic peptides corresponded to unique sequences within the metabotropic glutamate receptor type 2 and type 3 gene products, but was shared between the human (Q14416.2, Q14832.2 NCBI) and mouse (Q14B12.2, Q9QYS2.1, NCBI) sequences. After repeated injections, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, the concentrations of the eluates adjusted to 200 ug/ml, and the preparation was filter-sterilized.