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    Why Use Chicken IgY?

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    1. Higher titres against highly conserved mammalian gene products

    2. Double immunostaining is easier to perform

    3. Animal-friendly -- we purify the antibodies from eggs, not serum

    4. Large quantities of antibody - faster and cheaper

    5. Nearly unlimited quantities (again, because the antibodies come from eggs)

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Glutamate Receptor (Metabotropic) Type 2 (mGluR2)

Price: $399
Catalog #: ER2
Data Sheet (PDF)
mGluR2-staining of pyramidal neurons in the hippocampal formation of an adult transgenic mouse brain (red staining); Green staining is GFP-immunoreactivity for a subpopulation of cortical neurons.
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Product Info

Antibody Concentrations: 100 µg/ml anti-peptide antibodies (based on Bradford assay readings using bovine serum albumin as a standard)

Volume: Regular vials contain 1000 µl of this antibody preparation; Sampler vials contain 200 µl of this preparation.

Buffer: Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline (PBS, pH 7.2) with sodium azide (0.02%, w/v) added as a preservative.

Quality Control: This anti-peptide antibody preparation was validated by immunohistochemistry (at a concentration of 1 µg/ml) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.# F-1005) as the secondary reagent.

Recommended Dilutions: 1:1000-1:2000 for immunohistochemistry and immunocytochemistry using 2% paraformaldehyde-fixed tissues or cells. Note that these dilutions are meant to serve as starting points, and that optimal dilutions may vary.

Description: Chickens were immunized with a synthetic peptide / keyhole limpet hemocyanin (KLH) conjugate.  This synthetic peptide corresponded to a unique sequence within the metabotropic glutamate receptor type 2 gene product, but was shared between the human (Q14416.2, NCBI) and mouse (Q14B12.2, NCBI) sequences. After repeated injections, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, the concentrations of the eluates adjusted to 100 ug/ml, and the preparation was filter-sterilized.

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