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    Why Use Chicken IgY?

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    1. Higher titres against highly conserved mammalian gene products

    2. Double immunostaining is easier to perform

    3. Animal-friendly -- we purify the antibodies from eggs, not serum

    4. Large quantities of antibody - faster and cheaper

    5. Nearly unlimited quantities (again, because the antibodies come from eggs)

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Neu-N (Fox 3)

Price: $385
Catalog #: NUN
Data Sheet (PDF)
Neu-N (also known as Fox-3) Immunostaining.  Neu-N immunoreactivity (RED) showing the nuclei of granule cells in a section through an adult mouse cerebellum.  GREEN staining corresponds to rabbit antibodies against neurofilament heavy chain (NF-H) immunoreactivity.  Photomicrograph courtesy of Dr. Felix Eckenstein, University of Vermont.
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Product Info

Antibody Concentration:  100 µg/ml (based on Bradford assay readings using bovine serum albumin as a standard).

Volume: Regular vials contain 1000 ul; Sampler vials contain 200 ul of this mixture.

Buffer: Phosphate-buffered (10 mM) isotonic saline (0.9%, w.v) saline (PBS, pH 7.2) and sodium azide (0.02%, w/v).  The sodium azide is added as a preservative.

Quality Control: Immunohistochemistry (1:1000 dilution) using 2% paraformaldehyde-fixed tissues.

Recommended Dilutions: 1:1000-1:2000 for immunohistochemistry and immunocytochemistry using 2% paraformaldehyde-fixed tissues or cells. Note that these dilutions are meant to serve as a starting point, and that optimal dilutions may vary.

Description: Chickens were immunized with a synthetic peptide-keyhole limpet hemocyanin (KLH) conjugate.  This synthetic peptide corresponded to the Fox-3 gene product (also known as the Neu-N antigen), but was shared between the human NP_001076044, NCBI) and mouse (NP_001020102, NCBI) sequences. After repeated injections into the hens, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, the concentrations of the eluate adjusted to 100 ug/ml, and the preparation filter-sterilized.

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