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    Why Use Chicken IgY?

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    1. Higher titres against highly conserved mammalian gene products

    2. Double immunostaining is easier to perform

    3. Animal-friendly -- we purify the antibodies from eggs, not serum

    4. Large quantities of antibody - faster and cheaper

    5. Nearly unlimited quantities (again, because the antibodies come from eggs)

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Price: $245
Catalog #: PER
Data Sheet (PDF)
Peripherin-positive axons (red) in cortical cultures from neonatal mice.
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Product Info

Antibody Concentrations: 2.0 mg/ml.

Volume: Regular vials contain 200 µl of this antibody preparation.

Buffer: Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline (PBS, pH 7.2) with sodium azide (0.02%, w/v) added as a preservative.

Quality: Control Antibodies were analyzed by immunohistochemistry (at a dilution of 1:5000) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.# F-1005) as the secondary reagent.

Recommended Dilutions: 1:2000-1:5000 for immunohistochemistry and immunocytochemistry using 2% paraformaldehyde-fixed tissues or cells. 1:5000-1:10000 for western blotting. Note that these dilutions are meant to serve as starting points, and that optimal dilutions may vary.

Description: Chickens were immunized with purified recombinant full-length rat peripherin protein (57 kDa). After repeated injections into the hens, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions are diluted 1:10 with 1% bovine serum albumin (to reduce absorption) in phosphate-buffered isotonic saline solution (PBS) with 0.02% sodium azide (as a preservative).