Resources
- Antibody Production 101
- Antibody Protocols
- Citrate Buffer Antigen Retrieval Protocol
- Antibody Storage Recommendations
- Advantages of Chicken IgY
- FAQ
- Aves’ Citations in the Literature
- Useful Links
How much antigen should I send?
For two hens (i.e., the minimum number we recommend), we suggest 4.0 mg in a total volume of 4.0 mls of PBS or TBS (i.e., 1.0 mg/ml). It is best if the protein is placed in four 1.0 ml aliquots and frozen on dry ice. If you only have a precious amount of protein, we can probably get by with a concentration of 0.5 mg/ml.