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    Why Use Chicken IgY?

  • Here are top 5 reasons:

    1. Higher titres against highly conserved mammalian gene products

    2. Double immunostaining is easier to perform

    3. Animal-friendly -- we purify the antibodies from eggs, not serum

    4. Large quantities of antibody - faster and cheaper

    5. Nearly unlimited quantities (again, because the antibodies come from eggs)

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What kind of column is used for the affinity purification?

We use agarose-based matrices for affinity purification. We find that such matrices provide the lowest non-specific binding and highest capacities. These matrices are chemically-modified to allow covalent attachment of antigen via either primary amine or sulfhydryl groups.