Aves Labs Services

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    Why Use Chicken IgY?

  • Here are top 5 reasons:

    1. Higher titres against highly conserved mammalian gene products

    2. Double immunostaining is easier to perform

    3. Animal-friendly -- we purify the antibodies from eggs, not serum

    4. Large quantities of antibody - faster and cheaper

    5. Nearly unlimited quantities (again, because the antibodies come from eggs)

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How can I be confident the anti-peptide antibodies will work?

The choice of peptide sequence for anti-peptide antibody production is the single most important step in this process. In this regard, we have over fifteen years of experience in this field and developed thousands of successful anti-peptide antibodies. Our Immunogenicity Algorithm® identifies sequences that involve analysis of hydrophilicity, chain flexibility, surface probability and the presence or absence of problematic and desirable residues in particular positions within the peptide. We also check our peptides for potential sites of post-translational modification, which would also reduce their binding properties. Finally, we run these peptides against a chicken database to avoid peptides with high homologies with endogenous chicken proteins. We guarantee that the peptides we identify will be immunogenic, and that the antibodies produced against them will recognize the cognate sequence. However, we cannot guarantee that these anti-peptide antibodies will recognize the native protein. This may be due to an unexpected post-translational modification within the sequence or to protein folding events which may hide the epitope.