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    Why Use Chicken IgY?

  • Here are top 5 reasons:

    1. Higher titres against highly conserved mammalian gene products

    2. Double immunostaining is easier to perform

    3. Animal-friendly -- we purify the antibodies from eggs, not serum

    4. Large quantities of antibody - faster and cheaper

    5. Nearly unlimited quantities (again, because the antibodies come from eggs)

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How does one store chicken antibodies?

Store them as you would for mammalian antibodies – i.e., aliquot the antibodies and store them in the refrigerator in the presence of anti-microbial agents. Aves Labs routinely includes 0.02% sodium azide in our custom antibody preparations, unless specifically requested not to. If you plan to use your antibodies over a period of 3-5years, you may want to dilute your antibodies 1:1 v/v with sterile glycerol and store them at -20°C. Storage at -80°C is only recommended when clients expect to keep their antibodies over a period longer than 5 years. Regardless, be sure to avoid repeated freezing and thawing. Remember that you lose about half of an antibody’s activity (chicken IgY’s or rabbit IgG’s) each time the antibodies are frozen.

IgY’s are quite stable under normal conditions. According to Larsson et al. (1993), “the authors have stored (IgY) preparations for 10 years at 4°C without any significant loss in antibody activity.” They also state “chicken antibodies also retained their activity after 6 months at room temperature or 1 month at 37°C.” Remember that circulating IgY’s in chickens have half-lives on the order of months, and that the normal core temperature of a chicken is about 41°C.