How does chicken IgY compare methodologically with mammalian IgG?

Chicken antibodies are functionally equivalent to rabbit and other mammalian antibodies in most biochemical and cellular applications, including Western Blot (using both alkaline phosphatase- and horseradish peroxidase-labeled antibodies), Immunocytochemistry, and even Immunoprecipitation. Moreover, the affinities of rabbit and chicken antibodies are comparable with Kd values typically in the 1-3 ng/ml range. Refer to our detailed protocols for these and other methods.