β-Actin Loading Control for Westerns using Nitrocellulose Membranes; 3 Lanes loaded with mouse muscle homogenate (5 ug total protein, 10 ug total protein, 20 ug total protein); 1' antibody -- ACT (1:1000); 2' antibody -- HRP Goat anti-chicken IgY (Aves Labs, 1:2000)

β-Actin Loading Control for Westerns using Nitrocellulose Membranes; 3 Lanes loaded with mouse muscle homogenate (5 ug total protein, 10 ug total protein, 20 ug total protein); 1' antibody -- ACT (1:1000); 2' antibody -- HRP Goat anti-chicken IgY (Aves Labs, 1:2000)

Anti-Actin Antibody (Nitrocellulose Membrane Loading Control) (ACT)

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Details

Chickens were immunized with Actin purified from rabbit muscle. After repeated injections, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column. The final product contains 50% glycerol (v/v), purified anti-Actin IgY fraction (10 mg/ml) spiked with 25 µg/ml of affinity purified anti-Actin IgY, and then filter-sterilized.

Volume: 100 µL (ACT-1010) or 400 µL (ACT-1040)

Concentration: 10 mg/mL

Clonality: Polyclonal

Host Species: Chicken

Species Reactivity: Human, Mouse, Rat

Applications: WB

Antibody Registry ID (RRID): AB_2313504

Physical State: Liquid

Buffer: Sodium phosphate (10 mM, pH 7.2) buffered isotonic saline (0.9%, w/v), glycerol (50%, v/v), with sodium azide (0.02%, w/v) as an anti-microbial agent.

Validation and Application Notes

Expected Banding Pattern: 42 kDa

Quality Control

This anti-Actin IgY preparation was analyzed by Western Blot analysis (at a dilution of 1:2000) using HRP-labeled goat anti-chicken IgY (1:5000 dilution, Aves Labs Cat. # H-1004) as the secondary reagent.

Storage

Store at -20˚C in the dark. Under these conditions, the antibodies should have a shelf life of at least 12 months (provided they remain sterile). Do not freeze antibodies at -80˚C, unless you want to store them for longer periods of time. Note that each time an antibody preparation is frozen about half its binding activity is lost.

NOTE

Aves Labs products are intended for use as research laboratory reagents. They are not intended for use as diagnostic or therapeutic reagents in humans.


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