Western blot.  Left Lane -- Cultured normal mouse embryo fibroblasts were transfected with a plasmid containing the SAP1a cDNA fused with a c-myc epitope tag at its C-terminus.  Lane 1, 30 ug protein; lane 2, 20 ug protein; lane 3, 10 up protein; lane 4, 5 ug protein.

Western blot. Left Lane -- Cultured normal mouse embryo fibroblasts were transfected with a plasmid containing the SAP1a cDNA fused with a c-myc epitope tag at its C-terminus. Lane 1, 30 ug protein; lane 2, 20 ug protein; lane 3, 10 up protein; lane 4, 5 ug protein.

Anti-C-MYC Epitope Tag Antibody (ET-MY100)

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Product Details


Details

Chickens were immunized with a synthetic peptide containing the human c-myc epitope (i.e., EQKLISEEDL) conjugated to keyhole limpet hemocyanin. The first injection included complete Freund’s adjuvant; subsequent injections included incomplete Freund’s adjuvant. Eggs were collected after the fourth injection, and antibodies were purified from the yolks using a proprietary method that yields a purity of >90%. Antibodies were affinity-purified over a peptide column and then dialyzed against phosphate-buffered isotonic saline. Sodium azide was added and the antibodies were filter-sterilized (0.2 µm).

Volume: 1 mL

Concentration: 100 µg/mL

Clonality: Polyclonal

Form: Affinity Purified

Host Species: Chicken

Applications: ELISA, ICC, IHC, IP, WB

Antibody Registry ID (RRID): AB_2313514

Physical State: Liquid

Validation and Application Notes

Western Blot Dilution Range: 1:500-1:1000

IHC Dilution Range: 1:100-1:250

Quality Control

Antibodies were analyzed using ELISA and western blots using an N-terminal FLAG®-tagged protein. Secondary antibodies used in these quality control assays were alkaline phosphatase- (1:1000 dilution) and HRP-labeled (1:3000 dilution) goat anti-chicken IgY antibodies (Aves Labs), respectively.

Storage

Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least 12 months (provided they remain sterile). For longer storage periods, dilute the antibodies with sterile glycerol and store at -20°C. Do not freeze these antibodies unless you want to store them for longer periods of time.

NOTE

Aves Labs products are intended for use as research laboratory reagents. They are not intended for use as diagnostic or therapeutic reagents in humans.


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