Anti-DYKDDDDK Epitope Tag/FLAG Antibody

Anti-DYKDDDDK Epitope Tag/FLAG Antibody

Western blot.  Right Lane -- Cultured HEK cells were transfected with a plasmid containing the RDF1 cDNA fused with a FLAG epitope tag at its C-terminus.  Left Lane -- Cultured HEK cells with an empty plasmid vector.  25 ug of protein loaded in each lane.

Western blot. Right Lane -- Cultured HEK cells were transfected with a plasmid containing the RDF1 cDNA fused with a FLAG epitope tag at its C-terminus. Left Lane -- Cultured HEK cells with an empty plasmid vector. 25 ug of protein loaded in each lane.

Anti-DYKDDDDK Epitope Tag/FLAG Antibody (ET-DY100)

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Product Details


Details

Chickens were immunized with a synthetic peptide containing the DYKDDDDK epitope conjugated to keyhole limpet hemocyanin (KLH). The first injection included complete Freund’s adjuvant; subsequent injections included incomplete Freund’s adjuvant. Eggs were collected after the fourth injection, and antibodies were purified from the yolks using a proprietary method that yields a purity of >90%. Antibodies were then affinity-purified over a peptide column and dialyzed against phosphate-buffered isotonic saline. Sodium azide was added, the protein concentration was adjusted to 100 mg/mL, and the antibodies were filter-sterilized (0.2 µm).

Volume: 1 mL

Concentration: 100 µg/mL

Clonality: Polyclonal

Form: Affinity Purified

Host Species: Chicken

Applications: ELISA, ICC, IHC, IP, WB

Antibody Registry ID (RRID): AB_2313510

Physical State: Liquid

Validation and Application Notes

Western Blot Dilution Range: 1:1000-1:2000

IHC Dilution Range: 1:500-1:1000

Quality Control

Antibodies were analyzed using ELISA and western blots using an N-terminal DYKDDDDK-tagged protein. Secondary antibodies used in these quality control assays was an HRP-labeled (1:5000 dilution) goat anti-chicken IgY antibodies (Aves Labs).

Storage

Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least 12 months (provided they remain sterile). For longer storage periods, dilute the antibodies with sterile glycerol and store at -20°C. Do not freeze these antibodies unless you want to store them for longer periods of time.

NOTE

Aves Labs products are intended for use as research laboratory reagents. They are not intended for use as diagnostic or therapeutic reagents in humans.


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