Immunohistochemical staining of MBP (RED) in the hippocampal region of a transgenic adult mouse brain. This particular transgenic mouse expresses low levels of GFP autofluorescence until control of an actin promoter region. The anti-MBP antibody (Aves Labs) was used at a 1:1000 dilution. Dr. Felix Eckenstein, University of Vermont.

Immunohistochemical staining of MBP (RED) in the hippocampal region of a transgenic adult mouse brain. This particular transgenic mouse expresses low levels of GFP autofluorescence until control of an actin promoter region. The anti-MBP antibody (Aves Labs) was used at a 1:1000 dilution. Dr. Felix Eckenstein, University of Vermont.

Immunohistochemical staining of the MBP (GREEN) in the nodes of Ranvier of the sciatic nerve of an adult rat. RED counterstaining is a ubliquitous gene product.  BLUE shows DAPI nuclear staining. Photomicrograph by Dr. Gerry Shaw (EnCorBio and University of Florida).

Immunohistochemical staining of the MBP (GREEN) in the nodes of Ranvier of the sciatic nerve of an adult rat. RED counterstaining is a ubliquitous gene product. BLUE shows DAPI nuclear staining. Photomicrograph by Dr. Gerry Shaw (EnCorBio and University of Florida).

Immunohistochemical staining of the MBP (GREEN) in the nodes of Ranvier of the sciatic nerve of an adult rat. RED counterstaining is a ubliquitous gene product.  BLUE shows DAPI nuclear staining. Photomicrograph by Dr. Gerry Shaw (EnCorBio and University of Florida).

Immunohistochemical staining of the MBP (GREEN) in the nodes of Ranvier of the sciatic nerve of an adult rat. RED counterstaining is a ubliquitous gene product. BLUE shows DAPI nuclear staining. Photomicrograph by Dr. Gerry Shaw (EnCorBio and University of Florida).

Immunohistochemical staining of the MBP (GREEN) in the nodes of Ranvier of the sciatic nerve of an adult rat. RED counterstaining is a ubliquitous gene product.  BLUE shows DAPI nuclear staining. Photomicrograph by Dr. Gerry Shaw (EnCorBio and University of Florida).

Immunohistochemical staining of the MBP (GREEN) in the nodes of Ranvier of the sciatic nerve of an adult rat. RED counterstaining is a ubliquitous gene product. BLUE shows DAPI nuclear staining. Photomicrograph by Dr. Gerry Shaw (EnCorBio and University of Florida).

Immunohistochemical staining of the MBP (GREEN) in the nodes of Ranvier of the sciatic nerve of an adult rat. RED counterstaining is a ubliquitous gene product.  BLUE shows DAPI nuclear staining. Photomicrograph by Dr. Gerry Shaw (EnCorBio and University of Florida).

Immunohistochemical staining of the MBP (GREEN) in the nodes of Ranvier of the sciatic nerve of an adult rat. RED counterstaining is a ubliquitous gene product. BLUE shows DAPI nuclear staining. Photomicrograph by Dr. Gerry Shaw (EnCorBio and University of Florida).

Immunohistochemical staining of the MBP (GREEN) in the nodes of Ranvier of the sciatic nerve of an adult rat. RED counterstaining is a ubliquitous gene product.  BLUE shows DAPI nuclear staining. Photomicrograph by Dr. Gerry Shaw (EnCorBio and University of Florida).

Immunohistochemical staining of the MBP (GREEN) in the nodes of Ranvier of the sciatic nerve of an adult rat. RED counterstaining is a ubliquitous gene product. BLUE shows DAPI nuclear staining. Photomicrograph by Dr. Gerry Shaw (EnCorBio and University of Florida).

Adult mouse cortical cultures were stained with chicken anti-MBP antibody (Aves Labs, GREEN, 1:1000 dilution) and rabbit anti-GFAP antibody (EnCorBio, RED; 1:1000 dilution). Photomicrograph by Dr. Gerry Shaw (EnCorBio and University of Florida).

Adult mouse cortical cultures were stained with chicken anti-MBP antibody (Aves Labs, GREEN, 1:1000 dilution) and rabbit anti-GFAP antibody (EnCorBio, RED; 1:1000 dilution). Photomicrograph by Dr. Gerry Shaw (EnCorBio and University of Florida).

MBP: frozen sections; all washes with Triton 0.5%; Region: Striatum; Blocking: BlockHen 1%; Secondary antibody: anti-chicken FITC (Aves); Epifluorescence microscope; 40x magnification.

MBP: frozen sections; all washes with Triton 0.5%; Region: Striatum; Blocking: BlockHen 1%; Secondary antibody: anti-chicken FITC (Aves); Epifluorescence microscope; 40x magnification.

Chicken anti-Myelin Basic Protein (MBP) (Green) and Mouse anti-GFAP (Red) with nuclei stained with Hoechst dye (Blue).

Chicken anti-Myelin Basic Protein (MBP) (Green) and Mouse anti-GFAP (Red) with nuclei stained with Hoechst dye (Blue).

Anti-Myelin Basic Protein (MBP)

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Details

Chickens were immunized with a synthetic peptide / keyhole limpet hemocyanin (KLH) conjugate. The peptide corresponded to a region of the MBP gene product shared between the human (NP_002376, NCBI) and mouse (NP_034907, NCBI) sequences. After repeated injections, immune eggs were collected, the IgY fractions were purified from the yolks, and then affinity-purified using a peptide column. The concentrations of the eluates were then adjusted to 100 µg/mL, and the preparation was filter-sterilized.

Volume: 1000 µL

Concentration: 100 µg/mL

Clonality: Polyclonal

Host Species: Chicken

Species Reactivity: Human, Mouse, Rat

Applications: ICC, IHC, WB

Protein Name / Synonyms: Myelin basic protein (MBP) (Myelin A1 protein) (Myelin membrane encephalitogenic protein)

Gene ID: MBP

Antibody Registry ID (RRID): AB_2313550

Physical State: Liquid

Validation and Application Notes

Expected Banding Pattern: Can recognize multiple bands between 14-22 kDa, corresponding to different MBP isoforms

Western Blot Dilution Range: 1:1000-1:2000

IHC Dilution Range: 1:1000-1:2000

Quality Control

Quality assurance analysis was performed using immunohistochemistry (at a dilution of 1:2000) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.# F-1005) as the secondary reagent.

Storage

Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least 12 months (provided they remain sterile). Do not freeze these antibodies unless you want to store them for longer periods of time. Note, however, that each time an antibody preparation is frozen, about half of its binding activity is lost.

NOTE

Aves Labs products are intended for use as research laboratory reagents. They are not intended for use as diagnostic or therapeutic reagents in humans.


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