Immunohistochemistry photomicrograph of PLP immunoreactivity (GREEN; 1:1000 dilution) in a cryostat section of an embryonic (e18) mouse brain.  BLUE is DAPI nuclear counterstain.  Hoda Ilias, Aves Labs.

Immunohistochemistry photomicrograph of PLP immunoreactivity (GREEN; 1:1000 dilution) in a cryostat section of an embryonic (e18) mouse brain. BLUE is DAPI nuclear counterstain. Hoda Ilias, Aves Labs.

Same photo as above, plus a western blot showing a single band at the correct molecular weight.

Same photo as above, plus a western blot showing a single band at the correct molecular weight.

Anti-Proteolipid Protein (PLP)

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Details

Chickens were immunized with a synthetic peptide / keyhole limpet hemocyanin (KLH) conjugate. The sequence of the synthetic peptide was shared between the human and mouse gene products. After repeated injections into the hens, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, the concentration of the eluate adjusted to 100 µg/mL, and the preparation was filter sterilized.

Volume: 1000 µL

Concentration: 300 µg/mL

Clonality: Polyclonal

Host Species: Chicken

Species Reactivity: Human, Mouse, Rat

Applications: ICC, IHC

Protein Name / Synonyms: Myelin proteolipid protein (PLP) (Lipophilin)

Gene ID: PLP1 PLP

Antibody Registry ID (RRID): AB_2313560

Physical State: Liquid

Validation and Application Notes

Expected Banding Pattern: 30 kDa

Western Blot Dilution Range: 1:2000-1:5000

IHC Dilution Range: 1:2000-1:5000

Quality Control

Each of the three antibodies were analyzed by immunohistochemistry (at a dilution of 1:2000) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.# F-1005) as the secondary reagent.

Storage

Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least 12 months (provided they remain sterile). Do not freeze these antibodies unless you want to store them for longer periods of time. Note, however, that each time an antibody preparation is frozen, about half of its binding activity is lost.

NOTE

Aves Labs products are intended for use as research laboratory reagents. They are not intended for use as diagnostic or therapeutic reagents in humans.


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Citations


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