Immunostaining of paraformaldehyde-fixed (4%) neonatal mouse brain cultures showing β-Tubulin 3 immunostaining (GREEN, Aves Labs, 1:1000 dilution). RED staining is rabbit-anti-GFAP (EnCor, 1:1000); BLUE is DAPI nuclear counterstain. Secondary antibodies were fluorescein-labeled goat anti-chicken IgY; Texas Red-labeled goat anti-rabbit IgG (both from Jackson ImmunoResearch). Dr. Gerry Shaw (EnCor Biotechnology).

Immunostaining of paraformaldehyde-fixed (4%) neonatal mouse brain cultures showing β-Tubulin 3 immunostaining (GREEN, Aves Labs, 1:1000 dilution). RED staining is rabbit-anti-GFAP (EnCor, 1:1000); BLUE is DAPI nuclear counterstain. Secondary antibodies were fluorescein-labeled goat anti-chicken IgY; Texas Red-labeled goat anti-rabbit IgG (both from Jackson ImmunoResearch). Dr. Gerry Shaw (EnCor Biotechnology).

Immunostaining of paraformaldehyde-fixed (4%) cultures of SpH64 neuroblastoma cells showing β-Tubulin 3 immunostaining (GREEN, Aves Labs, 1:1000 dilution). RED staining is rabbit-anti-actin (Rockland, 1:1000); BLUE is DAPI nuclear counterstain. Secondary antibodies were fluorescein-labeled goat anti-chicken IgY (Aves Labs, F-1005, 1:1000 dilution); Texas Red-labeled goat anti-rabbit IgG. Page Balisch (University of Arizona).

Immunostaining of paraformaldehyde-fixed (4%) cultures of SpH64 neuroblastoma cells showing β-Tubulin 3 immunostaining (GREEN, Aves Labs, 1:1000 dilution). RED staining is rabbit-anti-actin (Rockland, 1:1000); BLUE is DAPI nuclear counterstain. Secondary antibodies were fluorescein-labeled goat anti-chicken IgY (Aves Labs, F-1005, 1:1000 dilution); Texas Red-labeled goat anti-rabbit IgG. Page Balisch (University of Arizona).

Immunostaining of paraformaldehyde-fixed (4%) cultures of SpH64 neuroblastoma cells showing β-Tubulin 3 immunostaining (GREEN, Aves Labs, 1:1000 dilution). RED staining is rabbit-anti-actin (Rockland, 1:1000); BLUE is DAPI nuclear counterstain. Secondary antibodies were fluorescein-labeled goat anti-chicken IgY (Aves Labs, F-1005, 1:1000 dilution); Texas Red-labeled goat anti-rabbit IgG. Page Balisch (University of Arizona).

Immunostaining of paraformaldehyde-fixed (4%) cultures of SpH64 neuroblastoma cells showing β-Tubulin 3 immunostaining (GREEN, Aves Labs, 1:1000 dilution). RED staining is rabbit-anti-actin (Rockland, 1:1000); BLUE is DAPI nuclear counterstain. Secondary antibodies were fluorescein-labeled goat anti-chicken IgY (Aves Labs, F-1005, 1:1000 dilution); Texas Red-labeled goat anti-rabbit IgG. Page Balisch (University of Arizona).

Immunostaining of paraformaldehyde-fixed (4%) neonatal mouse brain cultures showing β-Tubulin 3 immunostaining (GREEN, Aves Labs, 1:1000 dilution). RED staining is rabbit-anti-GFAP (EnCor, 1:1000); BLUE is DAPI nuclear counterstain. Secondary antibodies were fluorescein-labeled goat anti-chicken IgY; Texas Red-labeled goat anti-rabbit IgG (both from Jackson ImmunoResearch). Dr. Gerry Shaw (EnCor Biotechnology).

Immunostaining of paraformaldehyde-fixed (4%) neonatal mouse brain cultures showing β-Tubulin 3 immunostaining (GREEN, Aves Labs, 1:1000 dilution). RED staining is rabbit-anti-GFAP (EnCor, 1:1000); BLUE is DAPI nuclear counterstain. Secondary antibodies were fluorescein-labeled goat anti-chicken IgY; Texas Red-labeled goat anti-rabbit IgG (both from Jackson ImmunoResearch). Dr. Gerry Shaw (EnCor Biotechnology).

β-Tubulin 3 Immunoblot using homogenates of various organs. Performed at Kingfisher Labs.

β-Tubulin 3 Immunoblot using homogenates of various organs. Performed at Kingfisher Labs.

β-Tubulin 3 Immunoblot using homogenates of adult mouse brain (1:3000 dilution).  Hoda Ilias, Aves Labs.

β-Tubulin 3 Immunoblot using homogenates of adult mouse brain (1:3000 dilution). Hoda Ilias, Aves Labs.

Anti-Beta-Tubulin 3 (TUJ)

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Details

Chickens were immunized with three synthetic peptide / keyhole limpet hemocyanin (KLH) conjugates. These synthetic peptides corresponded to different regions of the β-Tubulin 3 gene product, but are shared between the human (NP_AAL28094, NCBI) and rat (AAM28438, NCBI) protein sequences. After repeated injections, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, and the concentrations of the eluates adjusted to 300 µg/mL. Finally, equal volumes of each of the three affinity-purified anti-peptide antibodies were mixed, and the preparation was filter-sterilized.

Volume: 1000 µL

Concentration: 300 µg/mL

Clonality: Polyclonal

Host Species: Chicken

Species Reactivity: Human, Mouse, Rat

Applications: ICC, IHC, WB

Protein Name / Synonyms: Tubulin beta-3 chain (Tubulin beta-4 chain) (Tubulin beta-III)

Gene ID: TUBB3 TUBB4

Antibody Registry ID (RRID): AB_2313564

Physical State: Liquid

Validation and Application Notes

Expected Banding Pattern: 50 kDa

Western Blot Dilution Range: 1:10000-1:20000

IHC Dilution Range: 1:2000-1:5000

Quality Control

Each of the three antibodies were analyzed by immunohistochemistry (at a dilution of 1:2000) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.# F-1005) as the secondary reagent.

Storage

Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least 12 months (provided they remain sterile). Do not freeze these antibodies unless you want to store them for longer periods of time. Note, however, that each time an antibody preparation is frozen, about half of its binding activity is lost.

NOTE

Aves Labs products are intended for use as research laboratory reagents. They are not intended for use as diagnostic or therapeutic reagents in humans.


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