Metabotropic glutamate receptor type 5 (mGluR5, 1:1000 dilution, Aves Labs Cat.# ER5) immunoreactivity (GREEN) and neurofilament-M (NF-M, Rockland, rabbit antibody, 1:500 dilution) immunoreactivity in a tissue section through an e18 mouse cochlear ganglion.

Metabotropic glutamate receptor type 5 (mGluR5, 1:1000 dilution, Aves Labs Cat.# ER5) immunoreactivity (GREEN) and neurofilament-M (NF-M, Rockland, rabbit antibody, 1:500 dilution) immunoreactivity in a tissue section through an e18 mouse cochlear ganglion.

Metabotropic glutamate receptor type 5 (mGluR5, 1:1000 dilution, Aves Labs Cat.# ER5) immunoreactivity (GREEN) and neurofilament-M (NF-M, Rockland, rabbit antibody, 1:500 dilution) immunoreactivity in a tissue section through the CA3 region of an e18 mouse hippocampal formation.

Metabotropic glutamate receptor type 5 (mGluR5, 1:1000 dilution, Aves Labs Cat.# ER5) immunoreactivity (GREEN) and neurofilament-M (NF-M, Rockland, rabbit antibody, 1:500 dilution) immunoreactivity in a tissue section through the CA3 region of an e18 mouse hippocampal formation.

Anti-Glutamate Receptor (Metabotropic) Type 5 (ER5)

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Product Details


Details

Chickens were immunized with three synthetic peptide / keyhole limpet hemocyanin (KLH) conjugates. These synthetic peptides corresponded to different regions of the metabotropic glutamate receptor type 5 gene product, but are shared between the human (NP_001137303.1, NCBI) and mouse (Q3UVX5.2, NCBI) sequences. After repeated injections, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, and the concentrations of the eluates adjusted to 300 µg/mL. Finally, equal volumes of these affinity-purified anti-peptide antibodies were mixed, and the preparation was filter-sterilized.

Volume: 1000 µL

Concentration: 300 µg/mL

Clonality: Polyclonal

Host Species: Chicken

Species Reactivity: Human, Mouse, Rat

Applications: ICC, IHC

Protein Name / Synonyms: Metabotropic glutamate receptor 5 (mGluR5)

Gene ID: GRM5 GPRC1E MGLUR5

Antibody Registry ID (RRID): AB_2313544

Physical State: Liquid

Validation and Application Notes

IHC Dilution Range: 1:1000-1:2000

Quality Control

This anti-peptide antibody mixture was analyzed by immunohistochemistry (at a dilution of 1:2000) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.# F-1005) as the secondary reagent.

Storage

Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least 12 months (provided they remain sterile). Do not freeze these antibodies unless you want to store them for longer periods of time. Note, however, that each time an antibody preparation is frozen, about half of its binding activity is lost.

NOTE

Aves Labs products are intended for use as research laboratory reagents. They are not intended for use as diagnostic or therapeutic reagents in humans.


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