Immunohistochemical phtomicrograph of adult mouse cerebral cortex stained for β-tubulin 3 (Rabbit antibody, GREEN; 1:500 dilution) and Neu-N (Chicken antibody from Aves Labs, RED, 1:1000 dilution).

Immunohistochemical phtomicrograph of adult mouse cerebral cortex stained for β-tubulin 3 (Rabbit antibody, GREEN; 1:500 dilution) and Neu-N (Chicken antibody from Aves Labs, RED, 1:1000 dilution).

Immunohistochemical phtomicrograph of adult mouse cerebral cortex stained for β-tubulin 3 (Rabbit antibody, GREEN; 1:500 dilution) and Neu-N (Chicken antibody from Aves Labs, RED, 1:1000 dilution).

Immunohistochemical phtomicrograph of adult mouse cerebral cortex stained for β-tubulin 3 (Rabbit antibody, GREEN; 1:500 dilution) and Neu-N (Chicken antibody from Aves Labs, RED, 1:1000 dilution).

Immunohistochemical photomicrograph of adult mouse hippocampal formation stained for β-tubulin 3 (Rabbit antibody, GREEN; 1:500 dilution) and Neu-N (Chicken antibody from Aves Labs, RED, 1:1000 dilution).  NOTE:  "HippocampusCA1.2.1B.jpg" is a blow up of the central section of this photomicrograph.

Immunohistochemical photomicrograph of adult mouse hippocampal formation stained for β-tubulin 3 (Rabbit antibody, GREEN; 1:500 dilution) and Neu-N (Chicken antibody from Aves Labs, RED, 1:1000 dilution). NOTE: "HippocampusCA1.2.1B.jpg" is a blow up of the central section of this photomicrograph.

Immunohistochemical photomicrograph of adult auditory ganglion stained for β-tubulin 3 (Rabbit antibody, GREEN; 1:500 dilution) and Neu-N (Chicken antibody from Aves Labs, RED, 1:1000 dilution).

Immunohistochemical photomicrograph of adult auditory ganglion stained for β-tubulin 3 (Rabbit antibody, GREEN; 1:500 dilution) and Neu-N (Chicken antibody from Aves Labs, RED, 1:1000 dilution).

Immunohistochemical photomicrograph of adult Organ of Corti stained for β-tubulin 3 (Rabbit antibody, GREEN; 1:500 dilution) and Neu-N (Chicken antibody from Aves Labs, RED, 1:1000 dilution).

Immunohistochemical photomicrograph of adult Organ of Corti stained for β-tubulin 3 (Rabbit antibody, GREEN; 1:500 dilution) and Neu-N (Chicken antibody from Aves Labs, RED, 1:1000 dilution).

Immunohistochemical photomicrograph of adult auditory ganglion stained for β-tubulin 3 (Rabbit antibody, 1:500 dilution) and Neu-N (Chicken antibody from Aves Labs, 1:1000 dilution).

Immunohistochemical photomicrograph of adult auditory ganglion stained for β-tubulin 3 (Rabbit antibody, 1:500 dilution) and Neu-N (Chicken antibody from Aves Labs, 1:1000 dilution).

Immunohistochemical photomicrograph of adult dentate gyrus of the hippocampal formation.  (Upper left) NeuN stains all of the granule cells (1:1000 dilution).  BrdU (bromodeoxyuridine) stains proliferating cells.  Note that some granules cells of the dentate gyrus were labeled with BrdU, indicating that they were in the G phase of their cell cycle when BrdU was available.  Felix Eckenstein, University of Vermont.

Immunohistochemical photomicrograph of adult dentate gyrus of the hippocampal formation. (Upper left) NeuN stains all of the granule cells (1:1000 dilution). BrdU (bromodeoxyuridine) stains proliferating cells. Note that some granules cells of the dentate gyrus were labeled with BrdU, indicating that they were in the G phase of their cell cycle when BrdU was available. Felix Eckenstein, University of Vermont.

Immunohistochemical photomicrograph of neurons in cerebral cortex from a patient with  Alzheimer's Disease.  Parafin-embedded section.  Anti-NeuN (Aves Labs, 1:1000 dilution); 2˚ antibody was H-1004 (HRP-labeled goat anti-chicken IgY, Aves Labs, 1:1000 dilution).

Immunohistochemical photomicrograph of neurons in cerebral cortex from a patient with Alzheimer's Disease. Parafin-embedded section. Anti-NeuN (Aves Labs, 1:1000 dilution); 2˚ antibody was H-1004 (HRP-labeled goat anti-chicken IgY, Aves Labs, 1:1000 dilution).

Anti-Neu-N (Fox 3) (NUN)

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Details

Hens were immunized with a synthetic peptide-keyhole limpet hemocyanin (KLH) conjugate. This synthetic peptide corresponded to the Fox-3 gene product (also known as the Neu-N antigen), but was shared between the human NP_001076044, NCBI) and mouse (NP_001020102, NCBI) sequences. After repeated injections into the hens, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, the concentrations of the eluate adjusted to 100 µg/mL, and the preparation filter-sterilized.

Volume: 1000 µL

Concentration: 100 µg/mL

Clonality: Polyclonal

Host Species: Chicken

Species Reactivity: Human, Mouse, Rat

Applications: ICC, IHC

Protein Name / Synonyms: RNA binding protein fox-1 homolog 3 (Fox-1 homolog C) (Neuronal nuclei antigen) (NeuN antigen)

Gene ID: RBFOX3

Antibody Registry ID (RRID): AB_2313556

Physical State: Liquid

Validation and Application Notes

Expected Banding Pattern: 46, 48 kDa

IHC Dilution Range: 1:1000-1:2000

Storage

Store at 4˚C in the dark. Under these conditions, the antibodies should have a shelf life of at least 12 months (provided they remain sterile). Do not freeze these antibodies unless you want to store them for longer periods of time. Note, however, that each time an antibody preparation is frozen, about half of its binding activity is lost.

NOTE

Aves Labs products are intended for use as research laboratory reagents. They are not intended for use as diagnostic or therapeutic reagents in humans.


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