Mouse fibroblast cultures transfected with a recombinant fragment of the mouse prion protein (green staining).  Non-infected cells show labeled nuclei (blue staining) due to treatment with Hoechst stain.

Mouse fibroblast cultures transfected with a recombinant fragment of the mouse prion protein (green staining). Non-infected cells show labeled nuclei (blue staining) due to treatment with Hoechst stain.

Anti-Prion Protein (PRN)

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Product Details


Details

Chickens were immunized with a recombinant fragment of the human prion protein emulsified with Freund’s complete and incomplete adjuvants.After multiple injections, eggs were collected from the hens, and IgY fractions were prepared from the yolks.Affinity-purified antibodies were then prepared using an agarose column to which the recombinant protein fragments were covalently attached. Finally, affinity purified antibodies (50 µg/mL final concentration) were mixed with the IgY fraction (10 mg/mL final concentration) in a mixture containing glycerol (50% v/v) (to prevent freezing at -20°C) and 0.02% sodium azide.

Volume: 200 µL

Concentration: 10 mg/mL

Clonality: Polyclonal

Form: Mixture of IgY fraction (10 mg/mL) and affinity-purified antibodies (50 µg/mL)

Host Species: Chicken

Species Reactivity: Human, Mouse, Rat

Applications: ICC, IHC, WB

Protein Name / Synonyms: Major prion protein (PrP) (ASCR) (PrP27-30) (PrP33-35C) (CD antigen CD230)

Gene ID: PRNP ALTPRP PRIP PRP

Antibody Registry ID (RRID): AB_2313559

Physical State: Liquid

Validation and Application Notes

IHC Dilution Range: 1:1000-1:2000

Quality Control

Antibodies were analyzed by immunocytochemistry (1:1000 dilution) using cells transfected with the prion protein cDNA.

Storage

Store at -20°C in the dark. Under these conditions, the antibodies should have a shelf life of at least 2 years (provided they remain sterile). For longer storage periods, store at -80°C. Note that storage at this lower temperature will destroy some antibody activity due to water crystallization.

NOTE

Aves Labs products are intended for use as research laboratory reagents. They are not intended for use as diagnostic or therapeutic reagents in humans.


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